Mis à jour: 11/26/2010

Course timetable - 2010

• Space_group
• Escher Web Sketch (official M.C. Escher symmetry sketches )
• Bragg's Law and Diffraction
• Fourier transform
• Crystal Structure Diffraction
  • The interactive course of crystallography
  • --> Crystallographic applets obtained from http://escher.epfl.ch/eCrystallography/ <--
• Notes on Electron Density, Phase Problem & Intensity Measurement
• Review of Diffraction Basics

Protein crystallization
• How to grow protein crystals
• Pictorial Library of Crystallization Drop Phenomena
• Atomic Force Microscopy(and see links therein)
  • Crystals at Birth
  • Growth of 3D Protein Molecules- Basics
  • Nucleation and Growth of Macromolecules

Cryo-crystallography
• How does Radiation Damage in Protein Crystals depend on X-ray Dose
• Heat transfer from protein crystals

X-ray Sources
• Synchrotron crystallography overview
• Strategies for macromolecular synchrotron crystallograph

Getting started
• 1 2 3 shoot
• Cryo-Notes from Bernard Rupp

Phasing
• The Patterson by Patterson
• Isomorphous Replacement
• Anomalous Scattering and Molecular Replacement

Modelling
• Model Building and Refinement

Links

• Ewald Sphere and Diffraction
• Protein Crystallographic Data Movies

1. Nuclear Magnetic Resonance Spectroscopy
2. NMR Relaxation
3. NMR Indirect Spin-Spin Coupling
4. NMR Parameters
5. Magnetization Transfer
6. NMR Pulse Sequences I
7. NMR Pulse Sequences II
8. NMR Spectroscopy Studies of Nucleic Acids
9. NMR Sequential Assignment Strategies
10. NMR Structure Calculations
11. NMR Chemical Exchange
12. Structure-based Drug Design

• Potential Energy Functions
• Energy Minimization & Molecular Dynamics
• Free Energy & Applications

The seminars will be held on Friday December 3rd, 2010 in D-560 at 11H30-15H00 . A Powerpoint or acetate presentation would be appreciated. Your presentation should last no more than 15 minutes leaving time for 5 minutes of questions. Please focus your presentation on the technique used in the article that you have chosen - whenever possible be ready to justify the use of this technique, why it was used and its advantages in the context of its application. Please consult the evaluation profile for which presentation has been assigned to you from the list of seminar topics.

1. Crystallography 101
2. Crystallographic facts
3. Protein Crystallography on the Web

1. Principles of Protein Crystallography by J. Drenth, Springer-Verlag Inc., New York
2. Practical Protein Crystallography by D. McRee, Academic Press Inc., San Diego